Introduction to Electron Microscopy for Biologists

Scanning Electron Microscopy at the sub-cellular level; Electron Probe X-ray microanalysis for the study of cell physiology; Preparation of cells and tissues for Immuno EM; Fixation, Freezing and Embedding for Genetically Altered Tissue; Combined video fluorescence and 3-D electron microscopy; From Live-cell Imaging to Scanning Electron Microscopy (SEM); Immunolabelling for High Resolution; Immunogoldlabeling of Thawed Cryosections; Close-to-Native Ultrastructural Preservation by High Pressure Freezing; High-Pressure Freezing and Low-Temperature Fixation of Cell Monolayers Grown on Sapphire Coverslips; Freeze-fracture Cytochemistry in Cell Biology; The Plasma membrane cytoskeleton; Correlated Light and Electron Microscopy of the Cytoskeleton; Electron microscopy of intermediate filaments; Studying Microtubules by Electron Microscopy; Endoplasmic reticulum and Golgi Complex; Visualization of Dynamins; Electron Microscopy of Collagen Fibril Structure In Vitro And In Vivo including Three-Dimensional Reconstruction; Visualisation of Desmosomes in the Electron Microscope; A Protocol for Isolation and Visualization of Yeast Nuclei by Scanning Electron Microscopy (SEM); High Pressure Freezing and Freeze Substitution of S.pombe and S.cerevisiae for TEM; Electron Microscopy of Lamin and the Nuclear Lamina in Caenorhabditis elegans; Visualization of Nuclear Organisation by Ultrastructural Cytochemistry; Scanning Electron Microscopy of Chromosomes; Infection at the Cellular Level; Electron Microscopy of Viruses and Virus-Cell Interactions.