Techniques in Confocal Microscopy

Part I. Practical Considerations and Equipment<br>1. Theoretical Basis of Confocal Microscopy<br>Robert H. Webb<br>2. Practical Considerations in Acquiring Biological Signals from Confocal Microscope<br>Hao He, Rose Chik Ying Ong, Kam Tai Chan, Ho Pui Ho, Siu Kai Kong<br>3. Equipment for Mass Storage and Processing of Data<br>Guy Cox<br>4. Antifading Agents for Confocal Fluorescence Microscopy<br>Miguel Berrios, Kimberly A. Conlon, and David E. Colflesh<br>5. Mounting Techniques for Confocal Microscopy<br>Manabu Kagayama and Yasuyuki Sasano<br>6. Preparation of Whole Mounts and Thick Sections for Confocal Microscopy<br>Hisashi Hashimoto, Hiroshi Ishikawa, and Moriaki Kusakabe<br>7. Use of Confocal Microscopy to Investigate Cell Structure and Function<br>Ghassan Bkaily, Danielle Jacques<br>8. Combining Laser Scanning Confocal Microscopy and Electron Microscopy<br>Xuejun Sun<br><br>Part II. Functional Approaches<br>9. Volume Measurements in Confocal Microscopy<br>Carlos B. Mantilla, Y.S. Prakash and Gary C. Sieck<br>10. Quantitation of Phagocytosis by Confocal Microscopy<br>George F. Babcock and Chad T Robinson MS<br>11. Receptor-Ligand Internalization<br>Guido Orlandini, Nicoletta Ronda, Rita Gatti, Gian Carlo Gazzola, and Alberico Borghetti<br>12. Quantitative Imaging of Metabolism by Two-Photon Excitation Microscopy<br>David W. Piston and Susan M. Knobel<br>13. Trafficking of the Androgen Receptor<br>Virginie Georget, Béatrice Terouanne, Jean-Claude Nicolas, and Charles Sultan<br>14. Localization of Proteases and Peptide Receptors by Confocal Microscopy<br>Kanti D. Bhoola, Celia J. Snyman and Carlos D. Figueroa<br>15. Multiphoton Excitation Microscopy, Confocal Microscopy, and Spectroscopy of Living Cells and Tissues: Functional Metabolic Imaging of Human Skin in Vivo<br>Barry R. Masters, Peter T. C. So, Ki Hean Kim, Christof Buehler, and Enrico Gratton<br>16. Video-Rate, Scannng Slit Confocal Microscopy of Living Human Cornea in Vivo: Three-Dimensional Confocal Microscopy of the Eye<br>Barry R. Masters and Matthias Böhnke<br>17. In Vivo Imaging of Mammalian Central Nervous System Neurons with the In Vivo Confocal Neuroimaging (ICON) Method<br>Sylvia Prilloff, Petra Henrich-Noack, Ralf Engelmann, Bernhard A. Sabel<br>18. Identification of Viral Infection by Confocal Microscopy<br>David N. Howell and Sara E. Miller<br>19. Membrane Trafficking<br>Sabine Kupzig, San San Lee, and George Banting<br><br>Part III. Green Fluorescent Protein<br>20. Monitoring of Protein Secretion with Green Fluorescent Protein<br>Christoph Kaether and Hans-Hermann Gerdes<br>21. Comparison of Enhanced Green Fluorescent Protein and Its Destabilized Form as Transcription Reporters<br>Xiaoning Zhao, Tommy Duong, Chiao-Chian Huang, Steven R. Kain, and Xianqiang Li<br>22. Measuring Protein Degradation with Green Fluorescent Protein<br>Stephen R. Cronin and Randolph Y. Hampton<br>23. Studying Nuclear Receptors with Green Fluorescent Protein Fusions<br>Gordon L. Hager<br>24. Signaling, Desensitization, and Trafficking of G Protein-Coupled Receptors Revealed by Green Fluorescent Protein Conjugates<br>Larry S. Barak, Jie Zhang, Stephen S. G. Ferguson, Stephane A. Laporte, and Marc G. Caron<br>25. Fluorescent Proteins in Single- and Multicolor Flow Cytometry<br>Lonnie Lybarger and Robert Chervenak<br>26. Jellyfish Green Fluorescent Protein: A Tool for Studying Ion Channels and Second-Messenger Signaling in Neurons<br>L. A. C. Blair, K. K.Bence, and J. Marshall<br>27. Expression of Green Fluorescent Protein and Inositol 1,4,5-Triphosphate Receptor in Xenopus laevis Oocytes<br>Atshushi Miyawaki, Julie M. Matheson, Lee G. Sayers, Akira Muto, Takayuki Michikawa, Teiichi Furuichi, and Katsuhiko Mikoshiba<br>28. Confocal Imaging of Ca2+, pH, Electrical Potential, and Membrane Permeability in Single Living Cells<br>John J. Lemasters, Donna R. Trollinger, Ting Qian, Wayne E. Cascio, and Hisayuki Ohata<br><br>Part IV. Laser Capture Microdissection<br>29. Laser Capture Microdissection and Its Applications in Genomics and Proteomics<br>James L. Wittliff<br>30. Going in Vivo with Laser Microdissection<br>Anette Mayer, Monika Stich, Dieter Brocksch, Karin Schütze, and Georgia Lahr<br>31. Flourescence in Situ Hybridization of LCM-Isolated Nuclei from Paraffin Sections<br>Douglas J. Demetrick, Sabita K. Murthy, and Lisa M. DiFrancesco