Nuclear Pore Complexes and Nucleocytoplasmic Transport - Methods

<p>1. Fifty Years of Nuclear Pores and Nucleocytoplasmic Transport Studies: Multiple Tools Revealing Complex Rules<br>2. Imaging Metazoan Nuclear Pore Complexes by Field Emission Scanning Electron Microscopy<br>3. Imaging Yeast NPCs: From Classical Electron Microscopy to Immuno SEM<br>4. Exploring Nuclear Pore Complex Molecular Architecture by Immuno-Electron Microscopy using Xenopus Oocytes<br>5. Utilizing the Dyn2 Dimerization-Zipper as a Tool to Probe NPC Structure and Function<br>6. The use of Targeted Proteomics to Determine the Stoichiometry of Large Macromolecular Assemblies<br>7. A Pulse-Chase Epitope Labeling to Study Cellular Dynamics of Newly Synthesized Proteins: A Novel Strategy to Characterize NPC Biogenesis and Ribosome Maturation/Export<br>8. Analysis of Nuclear Reconstitution, Nuclear Envelope Assembly, and Nuclear Pore Assembly using Xenopus in vitro Assays<br>9. Xenopus in vitro Assays to Analyze the Function of Transmembrane Nucleoporins and Targeting of Inner Nuclear Membrane Proteins<br>10. Imaging the Assembly, Structure and Function of the Nuclear Pore Inside Cells<br>11. Cell-Fusion Method to Visualize Interphase Nuclear Pore (NPC) Formation<br>12. An in vitro System to Study Nuclear Envelope Breakdown<br>13. Modern Tools to Study Nuclear Pore Complexes and Nucleocytoplasmic Transport in Caenorhabditis elegans<br>14. Assessing Regulated Nuclear Transport in Saccharomyces cerevisiae<br>15. Analysis of Nucleocytoplasmic Transport in Digitonin-Permeabilized Cells under Different Cellular Conditions<br>16. Novel Approaches for the Identification of Nuclear Transport Receptor Substrates<br>17. NPC Mimics: Probing the Mechanism of Nucleocytoplasmic Transport<br>18. Analysis of RNA Transport in Xenopus Oocytes and Mammalian Cells<br>19. Strategies for Investigating Nuclear-Cytoplasmic tRNA Dynamics in Yeast and Mammalian Cells<br>20. Dissecting Ribosome Assembly and Transport in Budding Yeast<br>21. Approaches to Studying Subnuclear Organization and Gene-Nuclear Pore Interactions </p>