Methods of Adipose Tissue Biology Part B

1. Preparation and Differentiation of Mesenchymal Stem Cells from Ears of Adult Mice<br>2. 3-D Adipocyte Differentiation and Peri-Adipocyte Collagen Turnover<br>3. Differentiation of White and Brown Adipocytes from Human Pluripotent Stem Cells<br>4. Optimal Protocol for the Differentiation and Metabolic Analysis of Human Adipose Stromal Cells<br>5. Human Adipose Derived Stromal/Stem Cell Isolation, Culture and Osteogenic Differentiation<br>6. Analysis of Adipose Tissue Lipid Using Mass Spectrometry<br>7. Measurement of Long-Chain Fatty Acid Uptake into Adipocytes<br>8. Measurement of the Unfolded Protein Response (UPR) to Investigate its Role in Adipogenesis and Obesity<br>9. Application of Activity-Based Protein Profiling to Study Enzyme Function in Adipocytes<br>10. Measurement of Lipolysis<br>11. Measurement of Lipolysis Products Secreted by 3t3-L1 Adipocytes Using Microfluidics<br>12. Methods for Performing Lipidomics in White Adipose Tissue<br>13. Measuring Respiratory Activity of Adipocytes and Adipose Tissues in Real Time<br>14. Detecting Protein Carbonylation in Adipose Tissue and in Cultured Adipocytes<br>15. Use of Fluorescence Microscopy to Probe Intracellular Lipolysis<br>16. Evaluation of Protein Phosphorylation during Adipogenesis