Biophysical Methods in Cell Biology

1. Single-molecule Imaging of Cytoplasmic Dynein in vivo<br>Vaishnavi Ananthanarayanan and Iva M.Tolić<br>2. Single molecule imaging in live cell using gold nanoparticles<br>Cécile Leduc, Satyabrata Si, Jérémie J. Gautier, Zhenghong Gao, Edakkattuparambil S. Shibu,   Alexis Gautreau, Grégory Giannone, Laurent Cognet and Brahim Lounis<br>3. Quantitative Measurement of Transcription Dynamics in Living Cells<br>Adam M. Corrigan and Jonathan R. Chubb<br>4. An Easy-to-use Single-molecule Speckle Microscopy Enabling Nanometer-scale Flow and Wide-range Lifetime Measurement of Cellular Actin Filaments<br>Sawako Yamashiro, Hiroaki Mizuno, Naoki Watanabe<br>5. Dissecting Microtubule Structures by Laser Ablation<br>Franziska Decker and Jan Brugués<br>6. Quantifying Mitochondrial Content in Living Cells<br>Matheus Palhares Viana, Swee Lim, Susanne M. Rafelski<br>7. High-content 3D Multicolor Super-resolution Localization Microscopy<br>Pedro M Pereira, Pedro Almada, Ricardo Henriques<br>8. Superresolution Measurements in vivo: Imaging Drosophila Embryo by Photoactivated Localization Microscopy<br>Binh-An Truong Quang and Pierre-François Lenne<br>9. Refractive Index Measurements of Single, Spherical Cells Using Digital Holographic Microscopy<br>Mirjam Schürmann, Jana Scholze, Paul Müller, Chii J. Chan, Andrew E. Ekpenyong, Kevin J. Chalut, Jochen Guck<br>10. Construction, Imaging, and Analysis of FRET-Based Tension Sensors in Living Cells<br>Andrew S. LaCroix, Katheryn E. Rothenberg, Matthew E. Berginski, Aarti N. Urs, Brenton D. Hoffman<br>11. Single Cell Mechanics: the Parallel Plates Technique<br>Nathalie Bufi, Pauline Durand-Smet, Atef Asnacios<br>12. Atomic Force Microscopy-based Force Measurements on Animal Cells and Tissues<br>Hélène O. B. Gautier, Amelia J. Thompson, Sarra Achouri, David E. Koser, Kathrin Holtzmann, Emad Moeendarbary, and Kristian Franze<br>13. Measuring the Elasticity of Plant Cells with Atomic Force Microscopy<br>Siobhan A. Braybrook<br>14. Dual Pipette Aspiration: A Unique Tool For Studying Intercellular Adhesion<br>Maté Biro and Jean-Léon Maître<br>15. Measurement of Cell Traction Forces with ImageJ<br>Jean-Louis Martiel, Aldo Leal, Laetitia Kurzawa, Martial Balland, Irene Wang, Timothée Vignaud, Qingzong Tseng, Manuel Théry <br>16. Micropillar Substrates: a Tool for Studying Cell Mechanobiology<br>Mukund Gupta, Leyla Kocgozlu, Bibhu Ranjan Sarangi, Felix Margadant, Mohammed Ashraf and Benoit Ladoux<br>17. Mapping Forces and Kinematics During Collective Cell Migration<br>Xavier Serra-Picamal, Vito Conte, Raimon Sunyer, José J. Muñoz, and Xavier Trepat<br>18. Practical Aspects of the Cellular Force Inference Toolkit (CellFIT)<br>Jim H. Veldhuis, David Mashburn, M. Shane Hutson, and G. Wayne Brodland<br>19. Quantification of Collagen Contraction in Three-dimensional Cell Culture<br>Matthias Bussonnier, Sara Geraldo, Anthony Simon, Danijela Vignjevic, Timo Betz<br>20. Generation of Biocompatible Droplets for in vivo and in vitro Measurement of Cell-generated Mechanical Stresses<br>Otger Campas<br>21. Laser Induced Wounding of the Plasma Membrane and Methods to Study the Repair Process<br>Ana Joaquina Jimenez, Paolo Maiuri, Julie Lafaurie-Janvore, Franck Perez and Matthieu Piel<br>22. Electrofusion of Giant Unilamellar Vesicles to Cells<br>Dikla Raz-Ben Aroush, Shlomit Yehudai-Resheff and Kinneret Keren<br>23. Measurement and Manipulation of Cell Size Parameters in Fission Yeast<br>Yonatan Zegman, Daria Bonazzi and Nicolas Minc<br>24. Methods for Rectifying Cell Motions in vitro: Breaking Symmetry Using Microfabrication and Microfluidics<br>J. Comelles, V. Hortigüela, E. Martínez and D. Riveline<br>25. Analyzing Bacterial Movements on Surfaces<br>E. Laura Munteanu, Ingrid Spielman, Nicolas Biais<br>26. Advances in Single Cell Experimental Design Made Possible by Automated Imaging Platforms with Feedback Through Segmentation<br>Alex J. Crick, Eugenia Cammarota, Katie Moulang, Jurij Kotar and Pietro Cicuta