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Mitochondrial Function

Gebonden Engels 2014 9780128014158
€ 194,75
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Samenvatting

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. Methods to assess mitochondrial function is of great interest to neuroscientists studying chronic forms of neurodegeneration, including Parkinson's, Alzheimer's, ALS, Huntington's and other triplet repeat diseases, but also to those working on acute conditions such as stroke and traumatic brain injury. This volume covers research methods on how to assess the life cycle of mitochondria including trafficking, fusion, fission, and degradation. Multiple perspectives on the complex and difficult problem of measurement of mitochondrial reactive oxygen species production with fluorescent indicators and techniques ranging in scope from measurements on isolated mitochondria to non-invasive imaging of metabolic function.

Specificaties

ISBN13:9780128014158
Taal:Engels
Bindwijze:Gebonden

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Inhoudsopgave

1. High-Content Functional Genomic Screening to Identify Novel Regulators of the PINK1-Parkin Pathway<br>Andy Cheuk-Him Ng, Stephen Baird and Robert A. Screaton<br>2. Measurement of Mitochondrial Turnover and Life Cycle using MitoTimer<br>Kyle M. Trudeau , Roberta A.Gottlieb and Orian S. Shirihai<br>3. Monitoring Mitophagy in Mammalian Cells<br>Yushan Zhu , Guo Chen, Linbo Chen, Du Feng, Lei Liu and Quan Chen<br>4. Photoactivatable Green Fluorescent Protein-Based Visualization and Quantification of Mitochondrial Fusion and Mitochondrial Network Complexity in Living Cells<br>Mariusz Karbowski, Megan M. Cleland and Brian Roelofs<br>5. Characterization of Mitochondrial Transport in Neurons<br>Bing Zhou, Mei-Yao Lin, Tao Sun, Adam L. Knight and Zu-Hang Sheng<br>6. Imaging of Mitochondrial Dynamics in Motor and Sensory Axons of Living Mice.<br>Irene Bolea, Wen-Biao Gan, Giovanni Manfedi and Jordi Magranél<br>7. Analyzing Mitochondrial Dynamics in Mouse Organotypic Slice Cultures<br>Anh H. Pham and David C. Chan<br>8. Analysis of Mitochondrial Traffic in Drosophila<br>Swathi Devireddy, Hyun Sung, Pin-Chao Liao, Elisabeth Garland-Kuntz and Peter J Hollenbeck<br>9. In Vivo Imaging of Mitochondria in Intact Zebrafish Larvae<br>Dominik Paquet , Gabriela Plucinska and Thomas Misgeld<br>10. The Use of MiniSOG in the Localization of Mitochondrial Proteins <br>Guy A. Perkins<br>11. Assessing the Function of Mitochondria-Associated ER Membranes<br>Estela Area-Gomez.<br>12. Measurement of ROS Homeostasis in Isolated Mitochondria<br>L. Tretter, and A. Ambrus<br>13. Use of Potentiometric Fluorophores in the Measurement of Mitochondrial Reactive Oxygen Species<br>Brian M. Polster , David G. Nicholls , Shealinna X. Ge and Brian A. Roelofs<br>14. Spatial, Temporal and Quantitative Manipulation of Intracellular Hydrogen Peroxide in Cultured Cells<br>Ishraq Alim, Renee E Haskew-Layton, Hossein Aleyasin, Hengchang Guo and Rajiv R Ratan<br>15. Biochemical and Biophysical Methods for Studying Mitochondrial Iron Metabolism<br>Gregory P. Holmes-Hampton, Wing-Hang Tong and Tracey A. Rouault<br>16. Analysis and Interpretation of Microplate-Based Oxygen Consumption and PH Data<br>Ajit S. Divakaruni, Alexander Paradyse1, David A. Ferrick, Anne N. Murphy ,and Martin Jastroch<br>17. Imaging Changes in the Cytosolic ATP-to-ADP ratio<br>Mathew Tantama and  Gary Yellen<br>18. The Use of MiniSOG in the Localization of Mitochondrial Proteins <br>Carlos T. Moraes<br>19. Induced Pluripotent Stem Cell Derived Models for MtDNA Diseases<br>Riikka H Hämäläinen<br>20. The Use of 18F-BCPP-EF as a PET Probe for Complex I Activity in the Brain<br>Hideo Tsukada<br>21. MR OEF Imaging in MELAS<br>Sheng Xie

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€ 194,75
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