1. Axonal Transport: The Orderly Motion of Axonal Structures<br>Mark M Black<br>2. Live-Cell Imaging of Neurofilament Transport in Cultured Neurons<br>Atsuko Uchida, Paula C. Monsma, J. Daniel Fenn, Anthony Brown<br>3. Axonal Actin in Action: Imaging Actin Dynamics in Neurons<br>Kelsey Ladt, Archan Ganguly, Subhojit Roy<br>4. Live Imaging of Microtubule Dynamics in Organotypic Hippocampal Slice Cultures<br>Philipp Schätzle, Lukas C. Kapitein, Casper C. Hoogenraad<br>5. Studying Neuronal Microtubule Organization and Microtubule-Associated Proteins Using Single Molecule Localization Microscopy<br>Anaël Chazeau, Eugene A. Katrukha, Casper C. Hoogenraad, Lukas C. Kapitein<br>6. The Use of Proteomic Analysis to Study Trafficking Defects in Axons<br>Xiaoqin Fu, Kristy J. Brown, Sree Rayavarapu, Kanneboyina Nagaraju, Judy S. Liu<br>7. Using siRNA to Study Microtubule-Related Proteins in Cultured Neurons<br>Lanfranco Leo, Wenqian Yu, Peter W. Baas<br>8. Optimization of Cerebellar Purkinje Neuron Cultures and Development of a Plasmid-Based Method for Purkinje Neuron-Specific, miRNA-Mediated Protein Knockdown<br>C. J. Alexander, J. A. Hammer III<br>9. Biochemical Analysis of Axon-Specific Phosphorylation Events Using Isolated Squid Axoplasms<br>Minsu Kang, Lisa Baker, Yuyu Song, Scott Brady, Gerardo Morfini<br>10. Analyzing Kinesin Motor Domain Translocation in Cultured Hippocampal Neurons<br>Rui Yang, Marvin Bentley, Chung-Fang Huang, Gary Banker<br>11. Imaging and Quantitative Analysis of Cytokinesis in Developing Brains of Kinesin-6 Mutant Mice<br>Kerstin M. Janisch, Noelle D. Dwyer<br>12. Live Cell Imaging of Cytoplasmic Dynein Movement in Transfected Embryonic Rat Neurons<br>Mitchell W. Ross, David J. Mitchell, John C. Cain, Kiev R. Blasier, K. Kevin Pfister<br>13. Live-Cell Imaging of Retrograde Transport Initiation in Primary Neurons<br>Jeffrey J. Nirschl, Erika L. F. Holzbaur<br>14. Methods to Identify and Analyze Gene Products Involved in Neuronal Intracellular Transport Using Drosophila<br>Amanda L. Neisch, Adam W. Avery, James B. Machamer, Min-gang Li, Thomas S. Hays<br>15. In vivo Analysis of Axonal Transport in Zebrafish<br>Catherine M Drerup, Alex V Nechiporuk<br>16. Fast Axonal Transport in Isolated Axoplasm from the Squid Giant Axon<br>Yuyu Song, Minsu Kang, Gerardo Morfini, Scott T. Brady<br>17. Cellular and Subcellular Imaging of Motor Protein-Based Behavior in Embryonic Rat Brain<br>Alexandre D. Baffet, Aurélie Carabalona, Tiago J. Dantas, David D. Doobin, Daniel J. Hu, Richard B. Vallee<br>18. Tracking Quantum-Dot Labeled Neurotropic Factors Transport Along Primary Neuronal Axons in Compartmental Microfluidic Chambers<br>Shani Gluska, Michael Chein, Nimrod Rotem, Ariel Ionescu, Eran Perlson<br>19. Studying Endosomes in Cultured Neurons by Live-Cell Imaging<br>Zofia M. Lasiecka, Bettina Winckler<br>20. Comparative Analysis of Axonal Transport Markers in Primary Mammalian Neurons<br>Eva Klinman, Erika L. F. Holzbaur<br>21. Imaging Organelle Transport in Primary Hippocampal Neurons Treated with Amyloid-β Oligomers<br>Kathlyn J. Gan, Michael A. Silverman<br>22. High Spatial and Temporal Resolution Imaging of Motor-dependent Transport in Neuronal and Nonneuronal Cells<br>Julie Yi, Noopur V. Khobrekar, Tiago J. Dantas, Jie Zhou, Richard B. Vallee<br>23. Isolation and Analyses of Axonal Ribonucleoprotein Complexes<br>Ella Doron-Mandel, Stefanie Alber, Juan A. Oses, Katalin F. Medzihradszky, Alma L. Burlingame, Mike Fainzilber, Jeffery L. Twiss, Seung Joon Le