Echinoderms Part B

<p>1. Fertilization<br>When sperm meets egg—Fifty years of surprises<br>David Epel<br>Early and later studies on action potential and fertilization potential of echinoderm oocytes and Ca<SUP>2+</SUP> response of mammalian oocytes<br>Shunichi Miyazaki<br>My research career on (mainly) sea urchins<br>Victor D. Vacquier</p> <p>2. Cytokenesis and Embryology<br>Echinoderm eggs as a model for discoveries in cell biology<br>David R. Burgess<br>Unlocking mechanisms of development through advances in tools<br>David McClay<br>From hemoglobin to urchin spicules<br>Fred Wilt</p> <p>3. Genomics and Morphogenesis<br>The causes of things<br>Robert D. Burke<br>A personal history of the echinoderm genome sequencing<br>R. Andrew Cameron</p> <p>Section 1 - Methods for genome and transcriptome analysis<br>1. Genomic resources for the study of echinoderm development and evolution<br>Gregory A. Cary, R. Andrew Cameron and Veronica F. Hinman<br>2. Methods for the experimental and computational analysis of gene regulatory networks in sea urchins<br>Isabelle S. Peter<br>3. Using ATAC-seq and RNA-seq to increase resolution in GRN connectivity<br>Elijah K. Lowe, Claudia Cuomo, Danila Voronov and Maria I. Arnone<br>4. Identifying gene expression from single cells to single genes<br>Nathalie Oulhen, Stephany Foster, Greg Wray and Gary Wessel<br>5. Multiplex cis-regulatory analysis<br>Jongmin Nam<br>6. Whole mount in situ hybridization techniques for analysis of the spatial distribution of mRNAs in sea urchin embryos and early larvae<br>Eric M. Erkenbrack, Jenifer C. Croce, Esther Miranda, Sujan Gautam, Marina Martinez-Bartolome, Shunsuke Yaguchi and Ryan C. Range<br>7. Techniques for analyzing gene expression using BAC-based reporter constructs<br>Katherine M. Buckley and Charles A. Ettensohn<br>8. Genome-wide analysis of chromatin accessibility using ATAC-seq<br>Tanvi Shashikant and Charles A. Ettensohn</p> <p>Section 2 - Genome-editing and proteomics<br>9. Expression of exogenous mRNAs to study gene function in echinoderm embryos<br>Maria Dolores Molina, Christian Gache and Thierry Lepagef<br>10. Trapping, tagging and tracking: Tools for the study of proteins during early development of the sea urchin<br>Michelle M. Roux-Osovitz, Kathy R. Foltz, Nathalie Oulhen and Gary Wessel<br>11. CRISPR/Cas9-mediated genome editing in sea urchins<br>Che-Yi Lin, Nathalie Oulhen, Gary Wessel and Yi-Hsien Su<br>12 .Analysis of microRNA functions<br>Carolyn Remsburg, Kalin Konrad, Nina Faye Sampilo and Jia L. Song<br>13. In vivo analysis of protein translation activity in sea urchin eggs and embryos<br>Héloïse Chassé, Sandrine Boulben, Virginie Glippa, Florian Pontheaux, Patrick Cormier and Julia Morales<br>14. Generation, expression and utilization of single-domain antibodies for in vivo protein localization and manipulation in sea urchin embryos<br>Catherine S. Schrankel, Tufan Gökirmak, Chang-Wook Lee, Geoffrey Chang and Amro Hamdoun</p> <p>Section 3 - Imaging of echinoderm embryos<br>15. Live-cell fluorescence imaging of echinoderm embryos<br>Silvia P. Sepúlveda-Ramírez, Leslie Toledo-Jacobo, Chelsea Garno, Debadrita Pal, Clara Ross, <br>Andrea Ellis and Charles B. Shuster<br>16. 3D　+　time imaging of normal and twin sea urchin embryos for the reconstruction of their cell lineage<br>Antonio Ortiz, Elena Kardash and Nadine Peyriéras<br>17. High resolution imaging of the cortex isolated from sea urchin eggs and embryos<br>J.H. Henson, Bakary Samasa and E.C. Burg<br>18. Spatially mapping gene expression in sea urchin primary mesenchyme cells<br>Daniel T. Zuch and Cynthia A. Bradham</p> <p>Section 4 - Methods for measurement of intracellular signals in eggs, sperm and embryos<br>19. Probing Ca<SUP>2+</SUP> release mechanisms using sea urchin egg homogenates<br>Yu Yuan, Gihan S. Gunaratne, Jonathan S. Marchant and Sandip Patel<br>20. Measuring voltage and ion concentrations in live embryos<br>Nahomie Rodriguez-Sastre, Christopher F. Thomas and Cynthia A. Bradham<br>21. Analysis of sperm chemotaxis<br>Héctor Vicente Ramírez-Gómez, Idán Tuval, Adán Guerrero and Alberto Darszon<br>22. Kinetic and photonic techniques to study chemotactic signaling in sea urchin sperm<br>Hussein Hamzeh, Luis Alvarez, Timo Strünker, Michelina Kierzek, Christoph Brenker, Parker E. Deal, Evan W. Miller, Reinhard Seifert and U. Benjamin Kaupp<br>23. Analysis of neural activity with fluorescent protein biosensors<br>Robert D. Burke and Shunsuke Yaguchi</p>