Methods in Kidney Cell Biology Part A

<p>1. Generation of primary cells from ADPKD and normal human kidneys<br>Darren P. Wallace and Gail A. Reif<br>2. Measurement of cytoplasmic and cilioplasmic calcium in a single living cell<br>Rinzhin T. Sherpa, Rajasekharreddy Pala, Ashraf M. Mohieldin and Surya M. Nauli<br>3. Application of physiological shear stress to renal tubular epithelial cells<br>Nicholas Ferrell, Ruben M. Sandoval, Bruce A. Molitoris, Paul Brakeman, Shuvo Roy and William H. Fissell<br>4. ADPKD cell proliferation and Cl<SUP>−</SUP>-dependent fluid secretion<br>Gail A. Reif and Darren P. Wallace<br>5. In vitro cyst formation of ADPKD cells<br>Madhulika Sharma, Gail A. Reif and Darren P. Wallace<br>6. Engineering kidney tissues for polycystic kidney disease modeling and drug discovery<br>Valerio Brizi, Valentina Benedetti, Angelo Michele Lavecchia and Christodoulos Xinaris<br>7. Differentiation of human kidney organoids from pluripotent stem cells<br>Nelly M. Cruz and Benjamin S. Freedman<br>8. Studying Na<SUP>+</SUP> and K<SUP>+</SUP> channels in aldosterone-sensitive distal nephrons<br>Jacques Teulon and Wen-Hui Wang<br>9. Metanephric organ culture<br>Robin L. Maser, Brenda S. Magenheimer and James P. Calvet<br>10. Ex vivo kidney slice preparations as a model system to study signaling cascades in kidney epithelial cells<br>Biagio Saitta, Michael F. Jalili, Hamidreza Zohoorkari, Renee Rao, Kenneth R. Hallows, Catherine J. Baty and Nuria M. Pastor-Soler<br>11. Analysis of primary cilia in renal tissue and cells<br>Luciane M. Silva, Wei Wang, Bailey A. Allard, Tana S. Pottorf, Damon T. Jacobs and Pamela V. Tran<br>12. Quantifying autophagic flux in kidney tissue using structured illumination microscopy<br>Kensei Taguchi, Bertha C. Elias, Subo Qian and Craig R. Brooks<br>13. Investigation of epigenetics in kidney cell biology<br>Linda Xiaoyan Li, Ewud Agborbesong, Lu Zhang and Xiaogang Li</p>