Mitochondria Biology

Part 1 - Isolation of Mitochondria and Analysis of Mitochondrial Compartments<br>1. Isolation of mitochondria from animal cells and yeast<br>Francesco Pallotti and Liza Pon<br>2. Isolation and characterization of mitochondria-associated ER membranes<br>Estela Area-Gomez<br>3. Import of proteins into mitochondria<br>Nikolaus Pfanner and Thomas Becker<br>4. Proximity labeling methods to assess protein-protein interactions in yeast mitochondria<br>Jie-Ning Yang and Liza Pon <p>Part 2 - Assays for Mitochondrial Metabolic Activities<br>5. Biochemical assays of TCA cycle and β-oxidation metabolites<br>Michael J. Bennett, Sheng Feng and Ann Saada (Reisch)<br>6. Biochemical assays of respiratory chain complex activity <br>Robert Taylor<br>7. Assessing mitochondrial bioenergetics in whole cells and isolated organelles by microplate respirometry and polarography<br>Erich Gnaiger and Orian Shirihai<br>8. Analyzing electron transport chain supercomplexes<br>Jose Antonio Enriquez<br>9. Assays of mitochondrial ATP synthesis in animal cells and tissues<br>Paolo Pinton and Hiromi Imamura<br>10. Visualization of mitochondrial membrane potential in mammalian cells<br>Noemi Esteras, Merel Adjobo-Hermans, Andray Abramov and Werner JH Koopman<br>11. Visualization of mitochondrial function in living cells using biosensors (SoNAR, ro-GFP, DiOC6/Tom70 mCherry; DHE; Mitosox)<br>Yi Yang and Liza Pon</p> <p>Part 3 - Assays for Other Mitochondrial Activities<br>12. Histochemical methods to measure respiratory chain activity and visualize mitochondria and mitochondrial gene products <br>Kurenai Tanji and Sandra Franco-Iborra<br>13. Analysis of mitochondrial phospholipids and cardiolipin<br>Michael Schlame and Estela Area-Gomez<br>14. Biosensors for Detection of Calcium and pH<br>Diego Destefani<br>15. Measurement of membrane permeability and the mitochondrial permeability transition <br>Paolo Bernardi and Michela Carraro</p> <p>Part 4 - Mitochondrial Genes and Gene Expression <br>16. Detection of mtDNA mutations<br>Ali Naini, Sara Shanske, Robert Gilkerson and Jiuhong Pang<br>17. Visualization of mitochondrial DNA replication<br>Agnel Sfeir<br>18. Cybrid technology <br>Carlos Moraes and Sandra Bacman<br>19. Manipulation of mitochondrial genes and mtDNA heteroplasmy<br>Carlos Moraes, Sandra Bacman and Michal Minczuk</p> <p>Part 5 - Assays for Mitochondrial Dynamics<br>20. Assays for mitochondrial fusion and fission <br>Laura Lackner, Jason Lee, Jason Mears and Suzanne Hoppins<br>21. Methods to visualize mitochondria and mitochondrial motility<br>Liza Pon, Theresa Swayne, Ryo Higuchi-Sanabria and Pin-Chao Liao<br>22. In vitro and in vivo detection of mitophagy in animal cells, mice, and yeast (Keima; red/green mitophagosomes)</p> <p>Appendices<br>A. Basic properties of mitochondria<br>Luis García-Rodríguez<br>B. Inhibitors of the respiratory chain and of ATP synthesis<br>Nanette Orme-Johnson and Eric A. Schon<br>C. Linearized maps of selected mitochondrial genomes<br>Eric Schon<br>D. Mitochondrial genetic codes in various organisms <br>Eric Schon<br>E. Gene products present in mitochondria of yeast and animal cells <br>Eric Schon</p>