Integrated Methods in Protein Biochemistry: Part C

1. NanoBiT-based methods to monitor the activation and modulation of RTKs <br>2. The interplay of G-protein βγ subunit and PLC-β enzyme in PIP2 hydrolysis and downstream signaling <br>3. Biochemical Analysis of Protein-Protein Interfaces underlying the regulation of Bacterial Secretion Systems <br>4. Probing the structure and function of N-acetylmannosamine-6-phosphate 2-epimerase <br>5. Spectroscopic analysis of cysteine dioxygenase: a mammalian thiol-dioxygenase <br>6. DeGlyPHER: MS-based analysis of viral spike N-glycoforms <br>7. Covalent protein painting: MS-based protein footprinting <br>8. Characterization of GPCR signaling complexes using negative-staining electron microscopy <br>9. Probing protein misfolding and dissociation with free electron laser <br>10. Optimized protocol for the characterization of Cas12a activities <br>11. Proximity proteomics for the identification and characterization of extracellular vesicles <br>12. Functional characterization of lytic polysaccharide monooxygenases (LPMOs) <br>13. Characterization of RRE domain in RiPP biosynthesis <br>14. The Preparation of Recombinant Arginyltransferase 1 (ATE1) for Structural and Biophyiscal Characterizations <br>15. Testing anti-cancer drugs with Holographic Incoherent-light-source Quantitative Phase Imaging <br>16. Gel-based sample preparation methods for top-down native/cross-linking mass spectrometry <br>17. Identification and characterization of natural products and their interacts with anti-mycobacterial drug target proteins <br>18. Quantitative cysteinome profiling with DIA-ABPP <br>19. High-throughput screening and evolution of carbohydrate-active enzymes <br>20. Regulation of enzymes with light using unnatural amino acids <br>21. An optimized protocol for modular cloning to rapidly assemble expression vectors for MS-based orthogonal interactomics studies <br>22. Nucleotide-linked resins to identify new nucleotide-binding proteins using proteomics <br>23. Phospho-proteomic analysis of immune cells expressing chimeric antigen receptors <br>24. Novel strategies for targeting ubiquitin conjugation machinery using synthetic molecules <br>25. Reconstitution of the MLL1 complex and probing methyltransferase activity <br>26. Probing cellular aspects of endocytosis and phagocytosis with super-resolution microscopy <br>27. Structural and functional analysis of mono-copper enzymes from fungal species <br>28. Structure, function and regulation of deubiquitinases and RNA-modifying proteins. <br>29. Electrophysiology of ion-transporting rhodopsins <br>30. Measuring protein post-translational modifications using the protein semi-synthesis <br>31. Assay design and analysis of human Uracil DNA Glycosylase (UNG2) <br>32. Structure and function of T-Cell Protein Tyrosine Phosphatase (TCPTP) <br>33. Gel-based sample preparation methods for top-down/native/cross-linking mass spectrometry <br>34. Global substrate profiling of post-translational modifying enzymes <br>35. Methods for measuring kinetic isotope effects on the hydroxylation of a peptide