Practical Handbook on Soil Protists

<p>Soil sampling, processing and storage.-&nbsp;Initial observation of protist from soil.-&nbsp;Abundance calculation of naked and testate amoebae from soil.-&nbsp;Abundance calculation of mycophagous amoebae.-&nbsp;Abundance calculation of ciliates from soilAbundance calculation of flagellates from soil.-&nbsp;Methodology to study species diversity of naked amoeba and testate amoeba.-&nbsp;Non-flooded Petri plate method to study the species diversity of flagellates.-&nbsp;Enumeration of testate amoeba through direct count from soil.-&nbsp;Most Probable Number (MPN) method for enumeration of soil protists.-&nbsp;Standard solutions and media used for isolation of soil protists.-&nbsp;Isolation techniques for amoeba.-&nbsp;Isolation of ciliates and flagellates from soil.-&nbsp;Isolation and Enumeration of Mycophagous Protist.-&nbsp;Media used for enrichment of soil protists.-&nbsp;Enrichment of naked amoebae species.-&nbsp;Enrichment of ciliates and flagellates.-&nbsp;Enrichment of mycophagous protists.-&nbsp;Mono-axenic cultivation of protists.-&nbsp;Axenic cultivation of soil protists.-&nbsp;Classical wet mount method for observing live protists.-&nbsp;SEM: Sample Preparation, Fixation and Staining of Protists.-&nbsp;TEM: Sample Preparation, Fixation and Staining of Protists.-&nbsp;Protargol staining technique for identification of soil protists.-FLUTAX Staining of cilliates.-&nbsp;DNA extraction from trophozoites and cysts using manual protocol.-&nbsp;DNA isolation from single ciliate isolate.-&nbsp;Species identification through sequencing.-&nbsp;DNA Barcoding techniques for Protist.-&nbsp;Unequivocal identification through fluorescence in situ hybridization (FISH) technique.-&nbsp;Isolation, DNA extraction, amplification, and gel electrophoresis of single-celled non-marine foraminifera (Rhizaria).-&nbsp;Isolation and cultivation and staining of Paramecium.-&nbsp;Isolation and cultivation of Euglena.</p>

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