Derivative Spectrophotometry and Electron Spin Resonance (ESR) Spectroscopy for Ecological and Biological Questions

<p>Preface</p><p>Introduction</p><p>Chapter 1. Bases of the derivative spectrophotometry </p><p>1.1 The main law of light absorption by a substance </p><p>1.1.1 Reasons of deviation from Bouguer’s law</p><p>1.2 Correctness and accuracy of spectrophotometric data </p><p>1.2.1 Main factors influencing on accuracy of spectrophotometric measurements </p><p>1.2.2 Difference (differential) spectrophotometry </p><p>1.2.3 Measurement errors of the difference spectrophotometry </p><p>1.3 Derivative spectrophotometry </p><p>1.3.1 Methods of derivative signal registration and diagrams of differential analyzers </p><p>1.3.2 Parameter optimization of the differentiating circuit </p><p>1.3.3 The derivative spectrophotometry of difference spectra </p><p>1.3.4 Method of the pulse amplitude-modulated fluorescence for the solution of ecologicalbiochemical problems </p><p> </p><p>Chapter 2. The derivative spectrophotometry method for analysis of biologically active substances </p><p>2.1 The derivative spectrophotometry for analysis of a number of guanidine preparations </p><p>2.2 Сhelating ability of 1,3-bis-((p-chlorobenzylidene)amino) guanidine: complexes with Ca²⁺and La³⁺ ions </p><p>2.3 The special features of the Ca²⁺binding by mono-, bis- and tris-substituted guanidine derivatives </p><p>2.4 Special features of interaction of bis-((chlorobenzylidene)amino)guanidine derivatives with Ca²⁺ depending on the chlorine atom position in the molecule </p><p>2.5 The specific character of Ca²⁺ interaction with ((benzylidene)amino)guanidine derivatives containig electron-donor or electron-acceptor substituters </p><p>2.6 Special features of calcium ions interaction with bis-((4-hydroxy-3-methoxybenzilidene)amino)guanidine and bis-((4-cyanobenzilidene)amino) guanidine </p><p>2.7 The proof of polycomponent composition of the promising antitumor drug "Ukrain" </p><p>2.8 Derived spectra application for the analysis of derived forms of nondepolarizing muscle relaxant tercuronium, of vitamins and hormones </p><p>2.8.1 Comparative analysis of tercuronium derivatives </p><p>2.8.2 The reasonability of derived spectra application for the analysis of commercial preparations of vitamins and hormones </p><p>2.9 The importance of the derivative spectrophotometry in modern studies of aromatic amino acids and proteins </p><p>2.9.1 Special features of derived spectra of phenylalanine, tyrosine and tryptophan </p><p>2.9.2 Special features of phenylalanine spectra change caused by influence of gamma radiation</p><p>2.9.3 Specific character of tyrosine spectra changes under influence of gamma-radiation </p><p>2.9.4 The character of tryptophan derived spectra change under influence of gamma-irradiation</p><p>2.9.5 The comparative characteristic of albumin denaturation spectral changes under thermal and radiation exposures </p><p>2.9.6 Changes of the gamma globulin optical spectra under γ – irradiation </p><p>2.9.7 Special features of γ-globulin spectra changes during γ-globulin denaturation caused by thermal and radiation exposure</p><p>2.9.8 The coupling of albumin derived spectra change with the determination accuracy of albumin / globulin coefficient for radiation injuries </p><p> </p><p>Chapter 3. Applicability of the DSHO method in the work with pigments of plants and animals </p><p>3.1 Derived spectra of high orders for some carotenoids </p><p>3.2 Neoxanthin as a probable key product of formation of α- and β- carotenoid derivatives </p><p>3.3 Metabolic transformations of labeled 14C- or 3H- carotene in animal tissues </p><p>3.4 Importance of the derivative spectrophotometry for study of alternative ways of carotenoids biosynthesis in Procaryota and Eucaryota </p><p>3.5 Possibility of participation of α-ketoglutaric acid funds in carotenoids biosynthesis in chloroplasts </p><p>3.6 Malic acid as the source for carotenoids synthesis in plants with С4-way of carbon in photosynthesis </p><p>3.7 Indication of the de-epoxidation reaction with help of derived spectra </p><p>3.7.1 Coupling of the de-epoxidation reaction of xanthophylls with change of DII spectra at λ = 460 – 470 nm</p><p>3.7.2 Capabilities of the derivative spectrophotometry for assessment of influence of poisons and herbicides as extreme factors of environment </p><p>3.7.3 Features of influence of photosystem inhibitors and of photophosphorylation </p><p>3.7.4 The coupling of xanthophylls transformations with chloroplast energetics </p><p>3.7.5 Assessment of character of radiation injuries of the photosynthetic apparatus in vivo with help of derived spectra of high orders </p><p>3.7.6 The derivative spectrophotometry for the analysis of pigments of blood and its state </p><p>3.7.7 About possibility of application of the method of differentiation of spectral curves to decoding of electrocardiograms for the analysis of heart activity </p><p> </p><p>Chapter 4. EPR spectroscopy for solution of some scientific real-world problems in biology, medicine and ecology </p><p>4.1 The phenomenon of magnetic resonance </p><p>4.2 EPR phenomenon </p><p>4.2.1 Induced electron quantum transitions in EPR phenomenon </p><p>4.2.2 Hyperfine electron-nuclear interaction in the EPR method </p><p>4.2.3 A stationary method of magnetic resonance signals detection </p><p>4.3 EPR- dosimetry</p><p>4.3.1 EPR-dosimetry of population </p><p>4.3.2 EPR-dosimetry of objects and territories</p><p>4.3.3 "Alanine" dosimetry </p><p>4.4 Detection of paramagnetic ions in water solutions at room temperature</p><p>4.5 EPR of paramagnetic ions in low-temperature water-acidic matrixes </p><p>4.6 Detection of impurities capable of being photooxidized, in water, with the usage of electron phototransfer reaction </p><p>4.7 Determination of deuterium concentration in water </p><p>4.8 Multiquantum processes in reactions of photosynthesis and a photosensitization </p><p>4.9 Resolution of overlapped spectra </p><p>4.10 Small-sized specialized EPR equipment </p><p>4.11 Measurement of dielectric properties of substances at frequencies 10 and 30 GHz </p><p> </p><p>Conclusion</p><p><p>References</p><p>Subject Index</p>